Description

Differential interference contrast or DIC is a technique that enables the observation of qualitative difference in contrast for samples with different thickness. In DIC, light from the source is polarized by a polarizer and split into two parallel beams with a slight distance in-between using a Nomarski prism. These beams are illuminated on the sample and the beams that passed through it are recombined. If there is a difference in length between the two beam paths, it causes interference, which can be transformed into intensity for observation.